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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.

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Related Experiment Video

Updated: May 14, 2026

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Simultaneous correlative scanning electron and high-NA fluorescence microscopy.

Nalan Liv1, A Christiaan Zonnevylle, Angela C Narvaez

  • 1Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands.

Plos One
|February 15, 2013
PubMed
Summary

Simultaneous correlative light and electron microscopy (SCLEM) reduces inspection times by illuminating samples concurrently. This novel approach enables quantitative analysis of large biological datasets, overcoming limitations of traditional correlative microscopy.

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Last Updated: May 14, 2026

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Area of Science:

  • Cell Biology
  • Microscopy Techniques

Background:

  • Correlative light and electron microscopy (CLEM) links protein fluorescence to cellular ultrastructure.
  • Current CLEM methods are hindered by complex procedures for region retrieval and integration.

Purpose of the Study:

  • To develop a streamlined approach for correlative microscopy.
  • To reduce experimental time and enable quantitative analysis of large datasets.

Main Methods:

  • Simultaneous CLEM (SCLEM) uses a single setup with a high numerical aperture epi-fluorescence microscope and scanning electron microscope.
  • Illuminating the same sample area concurrently eliminates the need for region retrieval.

Main Results:

  • SCLEM drastically reduces inspection times for correlative microscopy.
  • Demonstrated SCLEM for analyzing cell-cell connections and membrane protrusions in colon adenocarcinoma cells.
  • Successfully imaged tissue sections with both fluorescence and electron-dense staining.

Conclusions:

  • SCLEM offers a more efficient and quantitative method for correlative microscopy.
  • This technique facilitates the investigation of biological structure-function relationships at high resolution.