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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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Related Experiment Video

Updated: May 14, 2026

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis
10:31

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

Stallion sperm transcriptome comprises functionally coherent coding and regulatory RNAs as revealed by microarray

Pranab J Das1, Fiona McCarthy, Monika Vishnoi

  • 1Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, United States of America.

Plos One
|February 15, 2013
PubMed
Summary

This study characterizes stallion sperm RNA, revealing a complex, functionally coherent collection of coding and non-coding RNAs. These findings enhance understanding of sperm RNA significance and may lead to fertility biomarkers.

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RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
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Published on: March 4, 2020

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Last Updated: May 14, 2026

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis
10:31

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
10:00

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Published on: March 4, 2020

Area of Science:

  • Reproductive Biology
  • Genomics
  • Molecular Biology

Background:

  • Mature mammalian sperm contain complex RNA populations with poorly understood roles in reproduction.
  • Sperm RNAs may regulate spermatogenesis, fertilization, and early embryonic development.
  • Understanding stallion sperm RNA is crucial for identifying fertility biomarkers.

Purpose of the Study:

  • To perform the first global transcriptome characterization of fertile stallion sperm.
  • To elucidate the biological significance and functional coherence of sperm RNAs.
  • To lay the groundwork for discovering sperm-based biomarkers for stallion fertility.

Main Methods:

  • Equine whole-genome oligoarray analysis of sperm and testes RNA.
  • RNA sequencing (RNA-seq) of stallion sperm.
  • Bioinformatic analysis including transcript mapping, annotation, and Gene Ontology enrichment.

Main Results:

  • Identified 6,761 transcripts in stallion sperm, with 165 sperm-enriched and 155 differentially expressed compared to testes.
  • RNA-seq mapped 19,257 sequence tags to the equine genome, with highest density on ECA11, 12, and 13.
  • Annotated transcripts associated with fertilization functions (e.g., signal transduction, ion transport) and potential roles in early development.

Conclusions:

  • Stallion sperm possess a rich, selectively retained repertoire of coding and non-coding RNAs.
  • These RNAs are not random remnants but a functionally coherent collection relevant to fertilization and development.
  • This research advances the understanding of sperm RNA biology and biomarker discovery potential.