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Related Experiment Video

Updated: May 14, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Activatable rotor for quantifying lysosomal viscosity in living cells.

Lu Wang1, Yi Xiao, Wenming Tian

  • 1State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.

Journal of the American Chemical Society
|February 16, 2013
PubMed
Summary

Researchers created Lyso-V, a novel fluorescent probe for measuring lysosomal viscosity. This tool enables real-time monitoring of viscosity changes in live cells using advanced microscopy techniques.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Microscopy

Background:

  • Lysosomes are crucial cellular organelles involved in degradation.
  • Monitoring lysosomal function requires precise tools to measure internal conditions.
  • Lysosomal viscosity is a key indicator of lysosomal health and function.

Purpose of the Study:

  • To develop and characterize a novel fluorescent probe for lysosomal viscosity.
  • To establish Lyso-V as a reliable lysosomal tracer.
  • To demonstrate the real-time quantification of lysosomal viscosity changes in live cells.

Main Methods:

  • Development of Lyso-V, a lysosome-activated fluorescent probe.
  • Application of Lyso-V in laser confocal microscopy for high-resolution imaging.

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Last Updated: May 14, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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  • Utilizing fluorescence lifetime imaging microscopy (FLIM) for quantitative analysis.

    • Main Results:

    • Lyso-V exhibits ideal lysosomal tracer characteristics with high spatial and temporal resolution.
    • The probe demonstrates effective fluorescence activation within lysosomes.
    • Real-time quantification of lysosomal viscosity changes in live cells was achieved using Lyso-V and FLIM.

    Conclusions:

    • Lyso-V is the first fluorescent probe specifically designed for lysosomal viscosity.
    • Lyso-V serves as an excellent lysosomal tracer for advanced microscopy.
    • This probe enables practical, real-time measurement of lysosomal viscosity dynamics in live cells.