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Updated: May 14, 2026

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
Published on: November 29, 2014
Kinetic assessment of N-methyl-2-methoxypyridinium species as phosphonate anion methylating agents
Joseph J Topczewski1, Daniel M Quinn
1Department of Chemistry, The University of Iowa, Iowa City, Iowa 52242, USA.
Abstract:
Organophosphate nerve agents and pesticides are potent inhibitors of acetylcholinesterase (AChE). Although oxime nucleophiles can reactivate the AChE-phosphyl adduct, the adduct undergoes a reaction called aging. No compounds have been described that reactivate the aged-AChE adduct. A family of 2-methoxypyridinium species which reverse aging in a model system is presented. A kinetic study of this system, which includes an SAR analysis, demonstrates that the reaction is highly tunable based on the ring substituents.
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