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Perinatal xenohormone exposure impacts sweet preference and submandibular development in male rats
W Kouidhi1, R Bergès, C Tiffon
1Research Unit n° 01/UR/08-07, Faculty of Medicine Tunis, Tunis El Manar University, Tunis, Tunisia; UMR 1324 CSGA, INRA, Dijon, France; UMR 6265 CSGA, CNRS, Dijon, France; CSGA, University of Burgundy, Dijon, France.
Objective:
To determine the effect of perinatal exposure to low doses of genistein and/or vinclozolin on submandibular salivary gland (SSG) development in juvenile and adult male rats and to establish a link with sweet preference.
Material And Methods:
Female rats received orally (1 mg kg(-1) body weight/day) genistein and vinclozolin, alone or in combination, from the first gestational day up to weaning. Sweet preference was assessed at weaning and in adulthood in male offspring; submandibular glands were then collected to study the morphogenesis and mRNA expression of steroid receptors, growth factors and taste related proteins.
Results:
Exposure to genistein and/or vinclozolin resulted in a higher saccharin intake on postnatal day 25 (P < 0.05) linked to a higher number of pro-acinar cells (P < 0.01) and mRNA expression of progesterone receptor, growth factors and gustine (P < 0.01). These increases disappeared in adulthood, but mRNA expressions of sex hormone receptors and growth factors were strongly repressed in all treated groups (P < 0.01).
Conclusion:
Our findings confirm that the SSG are target for xenohormones and provide evidence that perinatal exposure to low doses of genistein and/or vinclozolin could simultaneously disrupt not only the salivary gland prepubertal development and sweet intake but also endocrine gene mRNA expression later in life.
