Simple, fast, and efficient process for producing and purifying trehalulose.

Area of Science:

• Biochemistry
• Enzymology
• Biotechnology

Background:

• Trehalose synthase (GTase) is an enzyme with potential applications in carbohydrate synthesis.
• Optimizing GTase activity and developing efficient purification methods are crucial for its industrial use.

Purpose of the Study:

• To characterize a novel property of recombinant trehalose synthase (GTase) from Thermus thermophilus HB-8.
• To develop a simple, fast, and efficient method for producing and purifying high-purity trehalulose.

Main Methods:

• Utilized recombinant trehalose synthase (GTase) from Thermus thermophilus HB-8 to catalyze trehalulose formation from sucrose.
• Employed Saccharomyces cerevisiae cells for selective removal of byproducts and purification of trehalulose.
• Applied centrifugation and ion-exchange chromatography for final trehalulose isolation.

Main Results:

• GTase catalyzed trehalulose formation from sucrose with approximately 81% yield at 65°C, independent of substrate concentration.
• The purification method achieved over 95% trehalulose recovery with high purity.
• The process demonstrated efficiency, speed, and low production costs.

Conclusions:

• Recombinant GTase exhibits a valuable property for trehalulose synthesis.
• The developed purification strategy is effective for obtaining high-purity trehalulose.
• This method significantly improves production efficiency and reduces costs for trehalulose manufacturing.