Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Endoscopic Studies I: Bronchoscopy and Thoracoscopy01:30

Endoscopic Studies I: Bronchoscopy and Thoracoscopy

Endoscopy is a non-surgical medical technique used to examine a person's internal organs and vessels. This lesson will focus on two types of endoscopic studies: bronchoscopy and thoracoscopy.
Bronchoscopy
Description
Bronchoscopy is a procedure that involves direct visualization of the larynx, trachea, and bronchi for diagnostic and therapeutic purposes. A flexible fiber optic or rigid bronchoscope is used to carry out the procedure. The fiber-optic bronchoscope is more frequently used due to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep-learning endomicroscope with large field-of-view and depth-of-field for real-time in vivo imaging of epithelial cancer hallmarks.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Hybrid endomicroscopic objective with monolithic multi-material achromatic triplet fabricated using two-photon lithography.

Applied optics·2026
Same author

Miniature 3D-printed rod-like refractive objective for endoscopic applications.

Journal of biomedical optics·2026
Same author

Snapshot hyperspectral imaging microscope enabled by cladded waveguide array fabricated with 2-photon additive manufacturing.

Biomedical optics express·2026
Same author

Dual-modality, deep-learning-enabled endomicroscope with large field-of-view and depth-of-field for real-time in vivo imaging of epithelial hallmarks of cancer.

bioRxiv : the preprint server for biology·2026
Same author

Fully 3D-printed endomicroscopic objective for two-photon, multi-wavelength excitation microscopy.

Biomedical optics express·2026

Related Experiment Video

Updated: May 14, 2026

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Achromatized endomicroscope objective for optical biopsy.

Matthew Kyrish1, Tomasz S Tkaczyk

  • 1Department of Bioengineering, Rice University, 6100 Main St, Houston, TX 77005, USA.

Biomedical Optics Express
|February 16, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a narrow, achromatized endomicroscope objective for improved optical biopsies. This prototype demonstrates potential for enhanced cancer diagnostics through detailed cellular imaging.

Keywords:
(080.3620) Lens system design(170.2520) Fluorescence microscopy(170.3880) Medical and biological imaging(220.0220) Optical design and fabrication(220.1920) Diamond machining

More Related Videos

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation
09:42

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Published on: August 26, 2014

Related Experiment Videos

Last Updated: May 14, 2026

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation
09:42

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Published on: August 26, 2014

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Optical Engineering

Background:

  • Current endomicroscope objectives lack the narrow aperture required for in-situ optical biopsies.
  • A need exists for advanced imaging tools to improve cancer diagnostics and patient outcomes.

Purpose of the Study:

  • To present a proof-of-concept for a narrow, achromatized endomicroscope objective.
  • To validate the optical design and performance of this novel objective for potential clinical applications.

Main Methods:

  • Fabrication of a plastic lens-based objective with a 0.9 mm clear aperture and 0.4 numerical aperture (NA).
  • Achromatization of the objective across the visible spectrum (452 nm to 623 nm).
  • Optical sectioning using structured illumination and fluorescence imaging of stained breast carcinoma cells.

Main Results:

  • The objective achieved an NA of 0.4 with a 0.9 mm aperture, suitable for biopsy needle integration.
  • Measured Strehl ratio of 0.74 ± 0.05 across a 250 μm field of view (FOV) indicates good optical quality.
  • Successful fluorescence imaging of cancer cells demonstrates the objective's capability for cellular-level diagnostics.

Conclusions:

  • The developed endomicroscope objective represents a significant advancement for in-vivo optical biopsies.
  • This technology holds promise for enhancing the accuracy and scope of cancer diagnostics.