Related Experiment Video
Updated: May 14, 2026

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
Published on: July 26, 2024
Structure and evolutionary origins of the CMG complex
Silvia Onesti1, Stuart A MacNeill
1Structural Biology Laboratory, Elettra-Sincrotrone Trieste, Area Science Park, Basovizza, Trieste 34149, Italy. silvia.onesti@elettra.trieste.it
Abstract:
The CMG (Cdc45-MCM-GINS) complex is the eukaryotic replicative helicase, the enzyme that unwinds double-stranded DNA at replication forks. All three components of the CMG complex are essential for its function, but only in the case of MCM, the molecular motor that harnesses the energy of ATP hydrolysis to catalyse strand separation, is that function clear. Here, we review current knowledge of the three-dimensional structure of the CMG complex and its components and highlight recent advances in our understanding of its evolutionary origins.
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