Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 14, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Multiparameter DNA content analysis identifies distinct groups in primary breast cancer.

J H S Dayal¹, M J Sales, W E Corver

¹Dundee Cancer Centre, Ninewells Hospital and Medical School, University of Dundee, Clinical Research Centre Building, Dundee DD1 9SY, UK.

British Journal of Cancer

|February 16, 2013

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enabling DCIS subtyping: leveraging foundation models for robust grading and molecular biomarker scoring.

NPJ breast cancer·2026

Same author

Enhancing clinical decision support with genomic tools in breast cancer: A Scottish perspective.

Breast (Edinburgh, Scotland)·2024

Same author

Bridging the age gap in breast cancer: cluster randomized trial of two decision support interventions for older women with operable breast cancer on quality of life, survival, decision quality, and treatment choices.

The British journal of surgery·2021

Same author

Identifying triggers for hidradenitis suppurativa flare: a patient survey.

The British journal of dermatology·2021

Same author

Using observations and source specific model tracers to characterize pollutant transport during FRAPPÉ and DISCOVER-AQ.

Journal of geophysical research. Atmospheres : JGR·2020

Same author

Erythropoietin modulates striatal antioxidant signalling to reduce neurodegeneration in a toxicant model of Parkinson's disease.

Molecular and cellular neurosciences·2020

Multiparametric flow cytometry accurately assesses breast cancer DNA content. Tumour DNA index correlates with specific biomarkers and high S-phase fraction indicates poor survival.

Area of Science:

Oncology
Biotechnology
Genomics

Background:

Multiparameter flow cytometry is a reliable method for determining tumour DNA content in formalin-fixed paraffin-embedded (FFPE) tissue.
This study investigated clinical and pathological associations of DNA content in primary breast cancer using an advanced multiparametric technique.

Purpose of the Study:

To examine the clinical and pathological associations of DNA content in primary breast cancer.
To utilize an improved multiparametric flow cytometry technique for DNA content analysis.

Main Methods:

Analyzed FFPE tissue from 201 primary breast cancers using multiparameter flow cytometry.
Categorized cancers by DNA content (DNA index - DI) and calculated S-phase fraction (SPF%) using ModFit software.
Employed Binary Logistic Regression and Cox's Regression for association analysis.

More Related Videos

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Related Experiment Videos

Last Updated: May 14, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Main Results:

Low DI tumors associated with progesterone receptor-positive status.
Intermediate DI tumors linked to p53 mutations; high DI tumors correlated with HER2-positive status.
High SPF% (≥10%) independently associated with poorer overall survival.

Conclusions:

Multiparametric flow analysis of FFPE tissue accurately assesses tumor DNA content.
Tumor DNA content and subpopulations correlate with prognostic biomarkers and potential therapy response.
DNA content alterations offer insights into mechanisms of clinically significant biomarker changes in breast cancer.