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Related Experiment Video

Updated: May 14, 2026

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
09:03

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

Published on: February 13, 2021

Imaging axonal transport in the rat visual pathway.

Carla J Abbott1, Tiffany E Choe, Theresa A Lusardi

  • 1Discoveries in Sight Research Laboratories, Devers Eye Institute, and Legacy Research Institute, Legacy Health, Portland, OR 97232, USA.

Biomedical Optics Express
|February 16, 2013
PubMed
Summary

Researchers developed a new method to track axonal transport in retinal ganglion cells using cholera toxin b-subunit (CTB). This technique monitors transport in vivo and confirms it relies on microtubules and active mechanisms.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Ophthalmology

Background:

  • Axonal transport is crucial for neuronal function and survival.
  • Understanding axonal transport mechanisms is vital for diagnosing and treating neurological disorders.
  • Current methods for studying axonal transport in the anterior visual pathway have limitations.

Purpose of the Study:

  • To develop and validate a novel technique for assaying axonal transport in retinal ganglion cells.
  • To monitor the in vivo transport of a tracer in the rodent anterior visual pathway.
  • To investigate the dependence of this transport on microtubules and active transport mechanisms.

Main Methods:

  • Injection of cholera toxin b-subunit conjugated to AlexaFluor488 (CTB) into the anterior chamber of rodent eyes.
Keywords:
(170.2655) Functional monitoring and imaging(170.3880) Medical and biological imaging

Related Experiment Videos

Last Updated: May 14, 2026

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
09:03

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

Published on: February 13, 2021

  • In vivo imaging using confocal scanning laser ophthalmoscopy.
  • Post-mortem microscopy and brain imaging.
  • Assessment of CTB transport sensitivity to colchicine, a microtubule-disrupting agent.

    • Main Results:

    • A reliable technique for assaying axonal transport in retinal ganglion cells was established.
    • In vivo monitoring of CTB transport in the rodent anterior visual pathway was achieved.
    • CTB transport was found to be sensitive to colchicine, indicating microtubule dependence.
    • Bulk transport rates were determined: approximately 80-90 mm/day (anterograde) and 160 mm/day (retrograde).

    Conclusions:

    • The developed CTB assay is effective for monitoring axonal transport in vivo.
    • Axonal transport in the anterior visual pathway is mediated by active transport mechanisms dependent on intact microtubules.
    • This technique provides a valuable tool for studying axonal transport in neurological and visual pathway research.