Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Anchoring Junctions01:03

Anchoring Junctions

Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Tight Junctions01:29

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microglia suppress Müller cell FGF1 and contribute to retinal ganglion cell degeneration in glaucoma.

Experimental eye research·2026
Same author

Dynamic chromatin accessibility reveals BrKAN2 as a key regulator of Chinese cabbage leaf heading.

Molecular horticulture·2026
Same author

Trichoderma harzianum F115 promotes the growth of multiple crops under multiple stress conditions.

Journal of applied microbiology·2026
Same author

Damage response and postnatal compensatory repair in the male offspring mouse reproductive system following gestational cadmium exposure.

Toxicology and applied pharmacology·2026
Same author

Regulation of gene expression by 5mC DNA methylation during the bolting process of cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i> L.).

Frontiers in plant science·2026
Same author

Determinants of Willingness to Receive Health Information From Neighborhood Food and Beauty Establishments: Cross-Sectional Study.

JMIR public health and surveillance·2026

Related Experiment Video

Updated: May 14, 2026

An Epithelial Abrasion Model for Studying Corneal Wound Healing
04:45

An Epithelial Abrasion Model for Studying Corneal Wound Healing

Published on: December 29, 2021

Integrin alpha v beta 6 contributes to maintaining corneal epithelial barrier function.

Huiling Guo1, Gaiping Du, Liqiang Wang

  • 1Department of Ophthalmology, Chinese PLA General Hospital, Beijing, China; Department of Ophthalmology, Chinese PLA 306 Hospital, Beijing, China.

Cell Biology International
|February 16, 2013
PubMed
Summary

Integrin alpha v beta 6 (avb6) is crucial for corneal epithelial barrier function by aiding endosome-lysosome fusion. Disrupting avb6 or using TNF-α increases barrier permeability, impacting corneal health.

More Related Videos

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing
07:28

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

Published on: July 10, 2018

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
06:46

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Published on: February 15, 2019

Related Experiment Videos

Last Updated: May 14, 2026

An Epithelial Abrasion Model for Studying Corneal Wound Healing
04:45

An Epithelial Abrasion Model for Studying Corneal Wound Healing

Published on: December 29, 2021

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing
07:28

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

Published on: July 10, 2018

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
06:46

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Published on: February 15, 2019

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Immunology

Background:

  • Corneal epithelial barrier dysfunction is linked to various eye disorders.
  • Integrin alpha v beta 6 (avb6) is a key cell surface receptor.
  • Understanding avb6's role in corneal health is vital.

Purpose of the Study:

  • To investigate the function of integrin alpha v beta 6 (avb6) in maintaining corneal epithelial barrier integrity.
  • To elucidate the mechanism by which avb6 influences corneal epithelial cells.
  • To assess the impact of avb6 modulation on barrier permeability.

Main Methods:

  • Immunocytochemistry was used to observe avb6 localization with endosomes/lysosomes in human corneal epithelial (HCE) cells.
  • Corneal epithelial barrier function was assessed using HCE cell monolayers in Transwell systems.
  • avb6 function was modulated via knockdown and exposure to Tumor Necrosis Factor-alpha (TNF-α).

Main Results:

  • avb6 was found to associate with endosomes in HCE cells.
  • avb6 knockdown disrupted endosome-lysosome fusion.
  • TNF-α binding to avb6 interfered with endosome-lysosome fusion, increasing epithelial permeability to ovalbumin.

Conclusions:

  • Integrin alpha v beta 6 (avb6) plays a critical role in endosome-lysosome fusion within corneal epithelial cells.
  • avb6 inhibition or TNF-α exposure compromises the corneal epithelial barrier.
  • avb6 is essential for maintaining corneal epithelial barrier function and preventing dysfunction.