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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
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Metabolite structure analysis by high-resolution MS: supporting drug-development studies.

David S Wagner1, Jill L Pirhalla, Gary D Bowers

  • 1Department of Drug Metabolism & Pharmacokinetics, GlaxoSmithKline, Five Moore Drive, Research Triangle Park, NC 27709, USA.

Bioanalysis
|February 19, 2013
PubMed
Summary

High-resolution mass spectrometry (HRMS) is crucial for identifying drug metabolites in biological samples. This review details HRMS instrumentation and methods for metabolite structure analysis in drug development.

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Last Updated: May 14, 2026

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11:25

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Published on: July 11, 2014

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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
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Published on: March 14, 2013

Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Biochemistry

Background:

  • Characterizing drug metabolites in biological samples is essential for understanding drug efficacy and safety.
  • Traditional analytical methods may lack the necessary resolution, accuracy, and sensitivity for complex matrices.
  • High-resolution mass spectrometry (HRMS) offers advanced capabilities for metabolite identification.

Purpose of the Study:

  • To provide an overview of high-resolution mass spectrometry (HRMS) terminology and instrumentation.
  • To highlight approaches for metabolite structure analysis using HRMS.
  • To emphasize the value of HRMS in drug metabolism studies.

Main Methods:

  • Review of high-resolution mass spectrometry (HRMS) instrumentation.
  • Discussion of metabolite structure elucidation techniques.
  • Case examples illustrating HRMS applications in drug metabolism.

Main Results:

  • HRMS provides high resolving power, mass accuracy, and sensitivity for metabolite characterization.
  • Various HRMS instruments are available, each with specific advantages.
  • Specific analytical strategies effectively address challenges in metabolite structure analysis.

Conclusions:

  • High-resolution mass spectrometry (HRMS) is indispensable for comprehensive drug metabolism studies.
  • The sensitivity and accuracy of HRMS enable detailed characterization of drug metabolites.
  • HRMS significantly advances the understanding of drug behavior in biological systems.