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Plaque production by arboviruses in Singh's Aedes albopictus cells
Abstract:
We report plaquing tests of 124 virus strains, mostly arboviruses of 21 serological groups, in Singh's line of Aedes albopictus cells. Thirty of these plaqued: all were arboviruses of six groups and were known or presumed to be mosquito borne. Failing to plaque were 86 strains of arboviruses, mostly tick borne, two strains of insect pathogens, and six animal viruses not classified as arboviruses. Among mosquito-borne agents, plaquing ability appeared related to serological classification. California group and most A-group viruses failed to plaque, but nearly all members of B and Bunyamwera groups readily plaqued. Within serological group B, 14 of 16 mosquito-borne agents plaqued, but none of 13 tick-borne or vector-unassociated viruses did so. Some implications of these results for recognition and classification of arboviruses are discussed.
Insights
Plaquing tests on Aedes albopictus cells showed that most mosquito-borne arboviruses could be identified. Plaquing ability varied by serological group, aiding in arbovirus classification and recognition.
Area of Science:
- Virology
- Cell Biology
- Entomology
Background:
- Arboviruses are a diverse group of viruses transmitted by arthropods, primarily mosquitoes and ticks.
- Accurate identification and classification of arboviruses are crucial for public health and disease control.
- Aedes albopictus cell lines offer a potential tool for studying arbovirus replication and characteristics.
Purpose of the Study:
- To evaluate the plaquing efficiency of various arbovirus strains in Aedes albopictus cells.
- To determine if plaquing ability correlates with arbovirus serological groups and transmission vectors.
- To explore the utility of this method for arbovirus recognition and classification.
Main Methods:
- Plaquing assays were performed on 124 virus strains, including various arboviruses, using Singh's Aedes albopictus cell line.
- Virus strains were categorized based on their serological groups and known or presumed vectors (mosquito-borne, tick-borne, etc.).
- Plaquing success or failure was recorded for each virus strain tested.
Main Results:
- Thirty of 124 tested virus strains successfully plaqued, all of which were mosquito-borne arboviruses from six serological groups.
- Eighty-six arbovirus strains, predominantly tick-borne, failed to plaque.
- Plaquing ability was strongly associated with serological classification; for example, California and A-group viruses generally failed to plaque, while B and Bunyamwera groups readily plaqued. Within serogroup B, mosquito-borne agents plaqued, but tick-borne ones did not.
Conclusions:
- Plaquing in Aedes albopictus cells can differentiate between certain arbovirus groups and transmission types.
- This method shows promise as a tool for the preliminary recognition and classification of arboviruses.
- The findings highlight the importance of vector association and serological relationships in arbovirus behavior within cell culture systems.