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Related Concept Videos

Types of Receptors: Internal Receptors01:07

Types of Receptors: Internal Receptors

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Many cellular signals are hydrophilic and cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind intracellular receptors that reside within the cell cytoplasm or nucleus. Many mammalian steroid hormones and nitric oxide (NO) gas use this cell signaling mechanism.
Similar to membrane-bound receptors, the binding of a ligand to the intracellular receptor of causes a conformational change in the...
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Types of Receptors: Cell Surface Receptors01:28

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Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
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Internal Receptors01:31

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Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
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G-protein Coupled Receptors01:21

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G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
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Enzyme-linked Receptors01:00

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Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
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Human complement receptor type 1/CD35 is an Epstein-Barr Virus receptor.

Javier G Ogembo1, Lakshmi Kannan, Ionita Ghiran

  • 1Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.

Cell Reports
|February 19, 2013
PubMed
Summary

Complement regulatory protein CD35 acts as a novel Epstein-Barr virus (EBV) receptor on B cells, distinct from the known CD21 receptor. This finding reveals a new role for CD35 in EBV infection and vaccine development.

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Area of Science:

  • Virology
  • Immunology
  • Cell Biology

Background:

  • Epstein-Barr virus (EBV) uses gp350/220 for attachment to B cells.
  • CD21 is the established EBV receptor, but EBV infection in CD21-deficient cells has been reported.
  • CD21 forms complexes with CD19 and CD35 on B cells; CD35 is often lost in cell lines.

Purpose of the Study:

  • To investigate the role of CD35 in Epstein-Barr virus (EBV) initial infection.
  • To determine if CD35 can function as an EBV receptor independently of CD21.

Main Methods:

  • Transduction of CD21-negative cell lines with CD35, CD21, or both.
  • Assessing gp350/220 binding to engineered cells.
  • Evaluating EBV latent infection in the presence of coexpressed HLA II.

Main Results:

  • Cells expressing CD35 alone bound EBV gp350/220.
  • CD35-mediated EBV infection occurred when HLA II was coexpressed.
  • CD35-dependent infection exhibited distinct temporal, biophysical, and structural characteristics compared to CD21-mediated infection.

Conclusions:

  • CD35 is identified as a functional EBV receptor, playing a significant role in primary B cell infection.
  • This discovery addresses questions regarding EBV tropism and highlights EBVgp350/220's importance for vaccine strategies.