HelioScan: a software framework for controlling in vivo microscopy setups with high hardware flexibility, functional...

Area of Science:

• Neuroscience
• Biophysics
• Microscopy

Background:

• Intravital microscopy, particularly for in vivo brain dynamics, often relies on custom hardware and software.
• Technological advancements necessitate adaptable control software for novel imaging techniques and developer extensibility.

Purpose of the Study:

• Introduce HelioScan, a LabVIEW-based software package designed as a flexible platform for in vivo microscopy control.
• Provide a framework for researchers and developers to implement innovative imaging techniques and extensions.

Main Methods:

• HelioScan utilizes a component-based architecture, allowing flexible assembly of microscope control software tailored to specific hardware and functional needs.
• Applications are assembled at run-time from configurable software components, facilitating parallel development and community contributions.

Main Results:

• Demonstrated HelioScan's versatility through various in vivo brain imaging modes, including intrinsic optical signal imaging and two-photon laser-scanning microscopy.
• Successfully implemented high-speed in vivo two-photon calcium imaging using acousto-optic deflectors and resonant scanners.

Conclusions:

• HelioScan offers a convenient and adaptable software framework for the in vivo imaging community.
• Its component-based design supports tailored solutions and efficient development of new microscopy control functionalities.