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Coding variants of TLR2 and TLR4 genes do not substantially contribute to prosthetic joint infection

Frantisek Mrazek1, Jiri Gallo, Anna Stahelova

  • 1Laboratory of Immunogenomics and Immunoproteomics, Department of Immunology, Faculty of Medicine and Dentistry, Palacky University, I. P. Pavlova str. 6, 775 20, Olomouc, Czech Republic. mrazekf@fnol.cz

Inflammation Research : Official Journal of the European Histamine Research Society ... [Et Al.]
|February 19, 2013
PubMed
Abstract

Insights

Genetic variants in Toll-like receptors (TLR)2 and TLR4 do not appear to increase the risk of prosthetic joint infection (PJI) after total joint arthroplasty (TJA). This genetic association study found no significant differences in TLR gene variants between patients with and without PJI.

Area of Science:

  • Immunogenetics
  • Orthopedic Surgery
  • Infectious Disease

Background:

  • Prosthetic joint infection (PJI) is a serious complication following total joint arthroplasty (TJA).
  • Toll-like receptors (TLR)2 and TLR4 play crucial roles in innate immunity and pathogen recognition.
  • Genetic predisposition to PJI is not fully understood.

Purpose of the Study:

  • To investigate the association between specific genetic variants of TLR2 and TLR4 and susceptibility to PJI.
  • To determine if coding polymorphisms in TLR2 and TLR4 influence the risk of developing PJI after TJA.

Main Methods:

  • A genetic association study involving 350 TJA patients (98 with PJI, 252 without PJI) and 189 healthy controls.
  • Genotyping of three missense polymorphisms in TLR2 (R753Q, rs5743708) and TLR4 (D299G, rs4986790; T399I, rs4986791) using TaqMan assay.
  • Comparison of allele and genotype frequencies between PJI patients and control groups.

Main Results:

  • Allele frequencies of investigated TLR2 and TLR4 polymorphisms in healthy Czech individuals were comparable to other Caucasian populations.
  • No significant differences were observed in the distribution of TLR2/TLR4 genotype alleles between patients with PJI and control groups (non-septic TJA patients or healthy individuals).

Conclusions:

  • Structural genetic variants of TLR2 and TLR4 receptors do not appear to be a major factor in the risk of developing prosthetic joint infection.
  • Further research may be needed to identify other genetic or environmental factors contributing to PJI susceptibility.

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