Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 14, 2026

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
11:34

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Published on: December 5, 2017

Bioanalysis: Heri, hodie, cras.

Pier Giorgio Righetti1

  • 1Miles Gloriosus Academy, Milan, Italy. righetti.pg@tiscali.it

Electrophoresis
|February 19, 2013
PubMed
Summary
This summary is machine-generated.

This review highlights recent advancements in bioanalysis, focusing on techniques like SDS electrophoresis, isoelectric focusing, and capillary electrophoresis. It emphasizes combinatorial peptide ligand libraries for identifying low-abundance proteins in proteomics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Holy Grail possibly made from clay.

Electrophoresis·2024
Same author

Count Dracula Resurrected: Proteomic Analysis of Vlad III the Impaler's Documents by EVA Technology and Mass Spectrometry.

Analytical chemistry·2023
Same author

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique.

International journal of molecular sciences·2023
Same author

Meta-proteomic analysis of two mammoth's trunks by EVA technology and high-resolution mass spectrometry for an indirect picture of their habitat and the characterization of the collagen type I, alpha-1 and alpha-2 sequence.

Amino acids·2022
Same author

Proteomics and metabolomics composition of the ink of a letter in a fragment of a Dead Sea Scroll from Cave 11 (P1032-Fr0).

Journal of proteomics·2021
Same author

Combinatorial peptides: A library that continuously probes low-abundance proteins.

Electrophoresis·2021
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Proteomics

Background:

  • This review celebrates a decade of bioanalysis issues in Electrophoresis.
  • It focuses on current advancements rather than historical or future predictions.

Purpose of the Study:

  • To summarize recent progress in key bioanalytical techniques.
  • To highlight the importance of specific methods in modern proteomics.

Main Methods:

  • Sodium dodecyl sulfate (SDS) electrophoresis
  • Isoelectric focusing (IEF) and carrier ampholyte chemistry
  • Two-dimensional (2D) maps
  • Capillary electrophoresis (CE) with microchip technology and specialized coatings
  • Combinatorial peptide ligand library (CPLL) chemistry and function

More Related Videos

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
08:28

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

Related Experiment Videos

Last Updated: May 14, 2026

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
11:34

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Published on: December 5, 2017

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
08:28

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

Main Results:

  • Recent progress in SDS electrophoresis and IEF is detailed.
  • Advances in 2D mapping and CE technologies, including microchip applications, are presented.
  • The utility of CPLLs in detecting and identifying low-abundance proteins is emphasized.

Conclusions:

  • Combinatorial peptide ligand libraries are crucial for proteome analysis.
  • The review provides a snapshot of current bioanalytical methodologies.
  • These techniques are fundamental for advancing protein identification and characterization.