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Related Experiment Video

Updated: May 14, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

High throughput cell cycle analysis using microfluidic image cytometry (μFIC).

Hyun Ju Yoo1, Jonghoon Park, Tae Hyun Yoon

  • 1Department of Chemistry, Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|February 19, 2013
PubMed
Summary
This summary is machine-generated.

We developed a high throughput cell cycle analysis using fluorescence-based microfluidic image cytometry (μFIC). This method offers advantages over traditional flow cytometry for drug screening and biological research.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Microfluidic image cytometry (μFIC) enables cytotoxicity assessment in controlled microenvironments.
  • Previous μFIC methods utilized morphology and MTT absorbance.
  • Cell cycle analysis is crucial for understanding drug effects and cellular processes.

Purpose of the Study:

  • To develop and evaluate a high throughput cell cycle analysis method using fluorescence-based μFIC.
  • To assess paclitaxel-induced cell cycle changes in HeLa cells using the developed μFIC platform.
  • To compare the performance of fluorescence-based μFIC with traditional flow cytometry.

Main Methods:

  • Fabrication and optimization of a microfluidic device featuring a concentration gradient generator (CGG) and eight cell culture channels.
  • Application of the μFIC device for analyzing paclitaxel-induced cell cycle alterations in HeLa cells.
  • Utilizing fluorescence-based detection for quantitative cell cycle analysis within microfluidic channels.

Main Results:

  • Fluorescence-based μFIC provided cell cycle data comparable to flow cytometry.
  • The developed μFIC method demonstrated higher throughput and lower cost compared to flow cytometry.
  • μFIC generated less toxic waste, indicating a more sustainable approach.

Conclusions:

  • Fluorescence-based μFIC is a viable and effective platform for high throughput cell cycle analysis.
  • μFIC offers significant advantages over flow cytometry for pharmaceutical and biological applications.
  • This technology has the potential to become a standard platform for cell cycle analysis.