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Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: May 14, 2026

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

Stem cell-based therapy for corneal epithelial reconstruction: present and future.

Johannes Menzel-Severing1, Friedrich E Kruse, Ursula Schlötzer-Schrehardt

  • 1Department of Ophthalmology, University of Erlangen-Nürnberg, Erlangen, Germany.

Canadian Journal of Ophthalmology. Journal Canadien D'Ophtalmologie
|February 20, 2013
PubMed
Summary

Cultured limbal epithelial transplantation (CLET) shows variable success for blinding limbal stem cell deficiency. Future improvements focus on xenobiotic-free systems and biofunctional scaffolds to enhance safety and efficacy.

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Isolation and Identification of Limbal Niche Cells
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Isolation and Identification of Limbal Niche Cells

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Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
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Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Published on: October 24, 2018

Related Experiment Videos

Last Updated: May 14, 2026

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

Isolation and Identification of Limbal Niche Cells
10:11

Isolation and Identification of Limbal Niche Cells

Published on: October 27, 2023

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
10:07

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Published on: October 24, 2018

Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Limbal stem cell deficiency (LSCD) is a severe ocular condition causing pain and vision loss.
  • Cultured limbal epithelial transplantation (CLET) is a common surgical approach for corneal reconstruction in LSCD patients.
  • Current CLET techniques exhibit variable clinical outcomes, necessitating advancements for improved efficacy.

Purpose of the Study:

  • To review recent developments and emerging trends in CLET.
  • To identify strategies for enhancing the safety and efficacy of CLET.
  • To discuss future directions in corneal stem cell therapy.

Main Methods:

  • Review of recent scientific literature and clinical studies on CLET.
  • Analysis of novel approaches in tissue engineering and cell therapy for ocular surface reconstruction.
  • Exploration of alternative autologous stem cell sources.

Main Results:

  • Transition towards xenobiotic-free culture systems is a key trend.
  • Development of novel biofunctional scaffolds aims to improve stem cell maintenance in vitro and post-transplantation.
  • Hair follicles and other tissues are being investigated as alternative autologous stem cell sources for bilateral disease.

Conclusions:

  • Advancements in tissue engineering and cell therapy hold promise for CLET.
  • Biofunctional scaffolds and xenobiotic-free systems may increase CLET safety and efficacy.
  • Further research into the fundamental factors governing limbal stem cell maintenance and differentiation is crucial for optimal clinical results.