Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

StrucNS reveals interaction-weighted network topology as the driving predictor of absolute stability of natural and de novo proteins.

bioRxiv : the preprint server for biology·2026
Same author

Engineering a β-Sheet Enables Bispecific Binding in Single VHH Domains.

ACS synthetic biology·2026
Same author

Engineering Murine Cross-Reactivity Into an Affibody to Human Death Receptor 5.

Biotechnology and bioengineering·2026
Same author

Engineered B7-H3 Binding in Modular Gp2 Miniproteins.

Biotechnology and bioengineering·2026
Same author

Engineering hyaluronic acid-binding cytokines for enhanced tumor retention and safety.

bioRxiv : the preprint server for biology·2026
Same author

Yeast as a tool for exploring disulfide-rich peptides.

FEMS yeast research·2025

Related Experiment Video

Updated: May 14, 2026

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Engineering fibronectin-based binding proteins by yeast surface display.

Tiffany F Chen1, Seymour de Picciotto, Benjamin J Hackel

  • 1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Methods in Enzymology
|February 21, 2013
PubMed
Summary
This summary is machine-generated.

Yeast surface display (YSD) enables engineering of fibronectin (Fn3) domains for antigen binding. This method isolates Fn3 binders, offering antibody-like capabilities in a small, stable format.

More Related Videos

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
10:54

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Published on: May 30, 2025

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

Related Experiment Videos

Last Updated: May 14, 2026

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
10:54

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Published on: May 30, 2025

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

Area of Science:

  • Biotechnology
  • Protein Engineering
  • Molecular Biology

Background:

  • Yeast surface display (YSD) utilizes agglutinin subunits (Aga1p and Aga2p) to present proteins on yeast.
  • Human 10th type III fibronectin (Fn3) domains are small, stable, and lack disulfide bonds.
  • Engineered Fn3 libraries mimic antibody CDR-H3 loops for enhanced binding.

Purpose of the Study:

  • To outline a Yeast Surface Display (YSD) methodology for isolating fibronectin (Fn3) binders.
  • To leverage Fn3 domains as antibody mimics for diverse antigen targeting.

Main Methods:

  • Engineering a YSD library of Fn3 domains with CDR-H3-like loops.
  • Varying loop lengths within Fn3 domains to optimize binding.
  • Utilizing YSD for the selection of Fn3 binders against various antigens.

Main Results:

  • Demonstrated successful engineering of Fn3 domains for improved binding ability.
  • Showcased the potential of Fn3 domains as stable, small alternatives to antibodies.
  • Established a YSD methodology for isolating specific Fn3-antigen binders.

Conclusions:

  • Yeast surface display is an effective platform for developing Fn3-based binders.
  • Engineered Fn3 domains offer a versatile and stable alternative to antibodies for antigen recognition.
  • The described YSD methodology facilitates the isolation of Fn3 binders for diverse applications.