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Updated: May 14, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Comparison of some salivary characteristics between children with and without early childhood caries
Ali Bagherian1, Gholamreza Asadikaram
1Department of Pediatric Dentistry, Dental School, Rafsanjan University of Medical Sciences, Rafsanjan, Kerman, Iran.
Background And Aim:
Early childhood caries (ECC) is one of the most common chronic childhood diseases. Saliva as a host factor plays an essential role in maintaining the integrity of oral structures. The aim of the present study was to compare resting salivary pH, buffering capacity, and secretory immunoglobulin A (sIgA), calcium, and phosphate concentrations between children with and without ECC.
Materials And Methods:
In this cross-sectional study, samples of unstimulated saliva of 90 children (45 in ECC group and 45 in caries-free group) were taken with Scully method. The pH and buffering capacity were determined by pH meter. sIgA, calcium, and phosphate concentrations were quantitated with ELISA, CPC photometric, and phosphomolybdate/UV methods.
Results:
The mean resting salivary pH was significantly higher among children without ECC and the buffering capacity was significantly better among this group (P = 0.002). The mean sIgA concentration was significantly higher among the ECC group (P = 0.015). There were no statistically significant differences between calcium and phosphate concentrations between the two groups.
Conclusion:
The higher mean resting salivary pH and better buffering capacity found in children without ECC are probably the contributing factors that protect against caries development; but further studies are needed to understand the effects of saliva and its characteristics and components on ECC.
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