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Updated: May 14, 2026

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
Published on: April 6, 2022
Point-of-use water disinfection using UV light-emitting diodes to reduce bacterial contamination
Kristina Y Nelson1, Dena W McMartin, Christopher K Yost
1Environmental Systems Engineering, University of Regina, 3737 Wascana Parkway, Regina, SK, S4S 0A2, Canada. kristina.nelson@sasktel.net
Abstract:
The treatment process described in this research explores the impact of exposing water samples containing fecal coliforms to the radiation produced by single ultraviolet (UV) light-emitting diodes (LEDs) operating at 265 nm. UV LEDs are long lasting, compact in size and produce more efficient light output than traditional mercury-vapour bulbs, making them ideal for application in point-of-use disinfection systems, such as in remote areas. In this study, contaminated water samples containing either a pure culture of Escherichia coli or tertiary effluent from the City of Regina Wastewater Treatment Plant were used to study the application and efficiency of using UV LEDs for water disinfection. The results indicate that bacterial inactivation was achieved in a time-dependent manner, with 1- and 2.5-log E. coli reductions in water following 20 and 50 min of UV LED exposure, respectively. Ultraviolet radiation was less effective in reducing coliform bacteria in wastewater samples due to the elevated turbidity levels. Further work remains to be completed to optimize the application of UV LEDs for point-of-use disinfection systems; however, the results from this study support that bacterial inactivation using UV LEDs is possible, meriting further future technological development of the LEDs.
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