Related Experiment Video

Updated: May 14, 2026

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

Oligonucleotide recombination enabled site-specific mutagenesis in bacteria.

Bryan M Swingle1

  • 1United States Department of Agriculture, Agricultural Research Service, Ithaca, NY, USA. Bryan.Swingle@ars.usda.gov

Methods in Molecular Biology (Clifton, N.J.)
|February 21, 2013
PubMed
Summary

This study introduces oligonucleotide recombination, a precise bacterial genome engineering method. This technique enables targeted DNA modifications in vivo without external genetic elements.

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