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Related Experiment Video

Updated: May 14, 2026

Generating Spheroids from Various Chondrocytes using Low-Adhesive Conditions under Gravity and Homemade Mini-Bioreactors
09:03

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WISP3-IGF1 interaction regulates chondrocyte hypertrophy.

Srinivasa Rao Repudi1, Milan Patra, Malini Sen

  • 1Cancer Biology and Inflammatory Disorder Division, Council of Scientific and Industrial Research - Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Kolkata 700 032, India.

Journal of Cell Science
|February 21, 2013
PubMed
Summary

Wnt induced secreted protein 3 (WISP3) preserves cartilage integrity by inhibiting insulin-like growth factor 1 (IGF1) signaling, preventing chondrocyte hypertrophy and skeletal development issues.

Keywords:
HypertrophyIGF1WISP3

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Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Published on: October 4, 2024

Area of Science:

  • Biochemistry
  • Cell Biology
  • Skeletal Biology

Background:

  • Wnt induced secreted protein 3 (WISP3) is crucial for skeletal development.
  • Mutations in WISP3 cause progressive pseudo rheumatoid dysplasia (PPRD), characterized by cartilage loss.
  • The molecular mechanisms of PPRD and WISP3 function in cartilage remain unclear.

Purpose of the Study:

  • To investigate the role of WISP3 in maintaining cartilage integrity.
  • To elucidate the molecular interactions of WISP3 in chondrocytes.

Main Methods:

  • Investigated WISP3 binding to insulin-like growth factor 1 (IGF1).
  • Assessed the impact of WISP3 on IGF1 secretion and downstream signaling pathways.
  • Analyzed WISP3's effect on chondrocyte hypertrophy markers.

Main Results:

  • WISP3 directly binds to IGF1 and inhibits its secretion.
  • WISP3 suppresses IGF1-induced collagen X expression, reactive oxygen species (ROS) accumulation, and alkaline phosphatase activity.
  • IGF1 and ROS positively regulate WISP3 expression, forming a feedback loop.

Conclusions:

  • WISP3 acts as a negative regulator of chondrocyte hypertrophy by modulating IGF1 signaling.
  • A WISP3-IGF1 regulatory loop maintains cartilage homeostasis.
  • Understanding this loop offers insights into PPRD pathogenesis and potential therapeutic targets.