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[Effect of soy protein on expression of SREBP-1 in rats]
Lili Ma1, Guiyuan Ji, Zhuoqin Jiang
1Department of Food Science, Guangdong Food and Drug Vocational Colleage, Guangzhou 510520, China. malili198015@yahoo.com.cn
Objective:
To explore the effects of soy protein on the expression of SREBP-1,2 in rats feeding normal and high-fat diet.
Methods:
Forty eight Sprague-Dawley SPF class rats were randomly divided into 4 groups matched by body weight, soy protein group, casein group, soy protein-high fat group, casein-high fat group. The experiment diets were fed for 28 d. Then the rats were sacrificed off cervical, determined serum levels of homone and the level of gene expression.
Results:
The concentration of serum insulin and the ratio of insulin/glucagon in the soy protein group were significantly lower than that of the casein group ((54.1 +/- 10.7) vs (63.5 +/- 16.9) pmol/L, P < 0.05, (2.33 +/- 0.75) vs (3.21 +/- 1.41), P < 0.05), the concentration of serum insulin and the ratio of insulin/glucagon in the soy protein high-fat group were significantly lower than that of the casein high-fat group ((46.3 +/- 9.6) vs (50.4 +/- 10.1) pmol/L, P < 0.05, (1.53 +/- 0.72) vs (2.92 +/-1.23), P < 0.05). The expression of SREBP-1,2 in soy protein group and soy protein high-fat group were significantly lower than that in casein group and casein high-fat group. The protein expression of SREBP-1 in soy protein group and soy protein high-fat group were significantly lower than that in casein group and casein high-fat group.
Conclusion:
Dietary soy protein intake may modulate serum lipid levels through influence the insulin level then infulence the expression of SREBP-1,2 mRNA.
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