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Updated: May 14, 2026

MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease
Published on: February 14, 2012
Quantitative imaging of a therapeutic peptide in biological tissue sections by MALDI MS
Nozomi Takai1, Yukari Tanaka, Ayahisa Watanabe
1Drug Metabolism & Pharmacokinetics, Drug Developmental Research Laboratories, Shionogi & Co. Ltd., 3-1-1 Futaba, Toyonaka, Osaka 561-0825, Japan. nozomi.takai@shionogi.co.jp
Background:
Therapeutic peptides and proteins are being increasingly explored as potential therapeutic agents for molecular-targeted therapy, and the requirement for quantitative bioanalytical tools for such molecules has been discussed.
Results:
The distribution of octreotide, a synthetic octapeptide analog of somatostatin, in the liver and kidney of mice administered with the analog was clearly visualized by MALDI-Imaging MS (IMS). The developed MALDI-IMS analytical method successfully quantified the amount of octreotide on tissue sections (accuracy was 76-127%) and the 2,5-dihydroxybenzoic acid-based normalization method was effective.
Conclusion:
The results of this study suggest that MALDI-IMS enables the quantification of an administered therapeutic peptide on biological tissue sections, as well as visualization of the in vivo distribution of the peptide.
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