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Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
In vitro anticandidal evaluation of novel highly functionalized bis cyclohexenone ethyl carboxylates
V Kanagarajan1, M R Ezhilarasi, D Bhakiaraj
1Synthetic Organic Chemistry Laboratory, Department of Chemistry, Annamalai University, Annamalainagar, Tamil Nadu, India. profmgk@yahoo.co.in
Objectives:
Novel highly functionalized bis cyclohexenone ethyl carboxylates 7-12 were designed, synthesized and their structures were elucidated by their elemental analysis, MS, FT-IR, one-dimensional 1H, and 13C NMR spectroscopic data.
Materials And Methods:
All the synthesized compounds 7-12 were tested for their in vitro antifungal activities against Candida sp. namely Candida albicans, Candida tropicalis, Candida glabrata, Candida parapsilosis, Candida dubliniensis and Candida krusei.
Results:
A close inspection of the in vitro anticandidal activity profile in differently electron withdrawing (-F, -Cl, and -Br) functional group and electron donating (CH3 and OCH3) substituted phenyl rings of novel highly functionalized bis cyclohexenone ethyl carboxylates 7-12 exerted strong anticandidal activity against all the tested Candida species. All the synthesized compounds 7-12 exhibited MIC value in the range of 6.25-200 μg/mL against all the tested Candida (C.) species.
Conclusions:
Compound 8 against C. albicans, 9,11 against C. glabrata, 8,10 against C. parapsilosis, 7,9 against C. dubliniensis, 8,10 against C. krusei exhibited excellent anticandidal activity at a MIC value of 6.25 μg/mL. Likewise compound 7, 9-11 against C. albicans, 8, 9, 11 against C. tropicalis, 8 against C. glabrata, 9 against C. parapsilosis, 10 against C. dubliniensis, 9 against C. krusei revealed superior activity at a MIC value of 12.5 μg/mL.
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