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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: May 14, 2026

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
07:56

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

Direct detection pays off for electron cryo-microscopy.

Nikolaus Grigorieff1

  • 1is at the Department of Biochemistry and the Howard Hughes Medical Institute, Brandeis University , Waltham , Untied States niko@brandeis.edu.

Elife
|February 22, 2013
PubMed
Summary
This summary is machine-generated.

New electron detectors and image processing reduce the number of cryo-electron microscopy (cryo-EM) images needed to determine macromolecular structures. This advancement significantly streamlines the structural biology workflow.

Keywords:
BayesianDirect electron detectorsElectron microscopyImage processingS. cerevisiaeT. thermophilusribosome

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Miniaturized Sample Preparation for Transmission Electron Microscopy
09:04

Miniaturized Sample Preparation for Transmission Electron Microscopy

Published on: July 27, 2018

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Last Updated: May 14, 2026

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
07:56

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

Miniaturized Sample Preparation for Transmission Electron Microscopy
09:04

Miniaturized Sample Preparation for Transmission Electron Microscopy

Published on: July 27, 2018

Area of Science:

  • Structural biology
  • Biophysics
  • Biochemistry

Background:

  • Determining macromolecular structures is crucial for understanding biological processes.
  • Single-particle cryo-electron microscopy (cryo-EM) is a powerful technique for structural determination.
  • Previous cryo-EM methods required hundreds of thousands of images.

Purpose of the Study:

  • To present advancements in electron detectors and image processing for cryo-EM.
  • To demonstrate a reduction in the required number of images for structural determination.

Main Methods:

  • Utilizing improved electron detector technology.
  • Implementing advanced image-processing algorithms.
  • Applying these to single-particle cryo-electron microscopy.

Main Results:

  • Macromolecular structures can now be determined using tens of thousands of images.
  • This represents a significant decrease from the hundreds of thousands previously required.
  • The improved methods enhance efficiency in structural biology.

Conclusions:

  • The new technologies lower the data requirements for cryo-EM.
  • This facilitates more accessible and efficient structure determination of macromolecules.
  • Advancements promise to accelerate discoveries in structural biology.