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Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

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Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples
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High-resolution environmental transmission electron microscopy: modeling and experimental verification.

Makoto Suzuki1, Toshie Yaguchi, Xiao Feng Zhang

  • 1Hitachi High-Technologies Corporation, 882 Ichige, Hitachinaka, Ibaraki 312-8504, Japan.

Microscopy (Oxford, England)
|February 22, 2013
PubMed
Summary

This study introduces quantitative modeling for environmental transmission electron microscopy (ETEM) imaging. The model explains electron scattering in gas cells, improving image quality analysis for advanced microscopy.

Keywords:
Monte Carlo simulationcontrast transfer functionelectron-gas interactionelectron-solid interactionenvironmental transmission electron microscopygas environmental cell

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Area of Science:

  • Materials Science
  • Physics
  • Chemistry

Background:

  • Environmental transmission electron microscopy (ETEM) enables imaging under gas pressure.
  • Gas and window membranes in ETEM gas cells cause electron scattering, affecting image quality.
  • Quantitative modeling is needed to understand and mitigate scattering effects.

Purpose of the Study:

  • To develop a quantitative model for high-resolution phase contrast ETEM imaging.
  • To explain electron scattering phenomena within the gas environmental cell (E-cell).
  • To provide a framework for evaluating ETEM image quality under gas pressure.

Main Methods:

  • Introduction of pre-specimen scattering object (PreSO) and post-specimen scattering object (PoSO) concepts.
  • Modeling electron scattering using contrast transfer functions (CTFs).
  • Comparison of theoretical models with experimental gas ETEM data.

Main Results:

  • The model accurately describes electron scattering from gas and window membranes.
  • PreSO preserves phase information, evaluated via averaged CTFs.
  • PoSO represents information loss, with unscattered electrons critical for image quality.

Conclusions:

  • The developed quantitative model aligns well with experimental ETEM results.
  • The approach provides insights into factors affecting gas ETEM image resolution.
  • The model's extension to aberration-corrected ETEM is feasible.