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Updated: May 14, 2026

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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
Published on: September 11, 2016
Evidence for nonpoliovirus enterovirus multiplication in L20B cells
Luis Sarmiento Pérez1, Pedro Más Lago, Rosa Palomera Puentes
1Instituto De Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba. sarmiento@ipk.sld.cu
Revista Cubana De Medicina Tropical
|February 23, 2013
Abstract:
Stool specimens collected from 1 515 healthy children following a mass vaccination campaign in Cuba were tested for poliovirus excretion using L20B cell lines. In spite of the selectivity of this cell line for polioviruses (117/129; 90.7%) some other nonpolio enteroviruses (12/129: 9.3%), such as coxsackie A virus types 4, 8 and 10, can grow in L20B cells.

