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Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Published on: March 10, 2020
[Antimicrobial resistance of bacteria causing diarrhea in children from Corrientes, Argentina]
Silvia E Balbachán1, Luis A Merino, Daniel E Merino
1Institute de Medicina Regional, Universidad Nacional del Nordeste, Chaco, Argentina. silviabalbachan@yahoo.com.ar
Insights
A study in Argentina found that Shigella and Salmonella were the main causes of bacterial gastroenteritis in young children. Multidrug-resistant strains of Shigella, particularly Shigella flexneri, were frequently identified, highlighting a growing public health concern.
Area of Science:
- Microbiology
- Infectious Diseases
- Public Health
Context:
- Acute diarrhea is a significant health concern in children under 5 years old.
- Understanding the etiology of bacterial gastroenteritis is crucial for public health interventions.
- This study focused on identifying enteropathogens in fecal samples from Corrientes, Argentina.
Purpose:
- To identify the causative agents of bacterial gastroenteritis in young children in Corrientes, Argentina.
- To determine the antimicrobial susceptibility patterns of isolated enteropathogens.
- To contribute to the knowledge of regional bacterial gastroenteritis epidemiology.
Summary:
- Fecal samples from 590 children with acute diarrhea were analyzed between January 2004 and April 2005.
- Salmonella spp. and Shigella spp. were identified, with Shigella spp. being more prevalent (67.4%).
- Shigella flexneri was the predominant Shigella species, and multidrug resistance was observed in both Shigella and Salmonella isolates.
Impact:
- Identified Shigella flexneri as a major cause of bacterial gastroenteritis in the studied pediatric population.
- Revealed the presence of multidrug-resistant Salmonella and Shigella strains, indicating a potential public health threat.
- Provides essential data for developing targeted treatment strategies and infection control measures in the region.
Abstract:
In Corrientes (Argentina), from January 2004 to April 2005, fecal samples from under 5 years-old children with acute diarrhea were collected; the stool samples were cultured onto selective media, and those colonies suspected to be Salmonella, Vibrio, Aeromonas, Shigella, or Escherichia coli O157 were identified. The antimicrobial susceptibility was assessed by the disk diffusion method. Among 590 samples, the 7.7% were positive (Salmonella spp 32.6% and Shigella spp 67.4%). Of 31 Shigella isolates, 81% were S. flexneri, and 19% were S. sonnei. S. flexneri serotype 2 was the more frequent. S. flexneri strains showed higher multidrug resistance than S. sonnei strains. Among the Salmonella enterica serotypes, S. typhimurium and S. newport were the most frequent. Two isolates of Salmonella presented with multidrug resistance. This paper was a contribution to the knowledge of bacterial gastroenteritis etiology in our region and it called the attention to the emergence of multidrug-resistant enteropathogenic strains.
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