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A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Techniques for analysis of plant phenolic compounds
Ali Khoddami1, Meredith A Wilkes, Thomas H Roberts
1Department of Plant and Food Sciences, University of Sydney, Sydney NSW 2006, Australia.
Molecules (Basel, Switzerland)
|February 23, 2013
Summary
This review covers methods for analyzing plant phenolic compounds, focusing on extraction, identification, and quantification techniques. It highlights advancements in analyzing these important medicinal and dietary molecules.
Area of Science:
- Phytochemistry
- Analytical Chemistry
- Plant Science
Background:
- Phenolic compounds are diverse plant-derived phytochemicals with significant medicinal and dietary applications.
- Research on phenolic compounds has advanced considerably over the past 25 years, particularly in their extraction and analysis.
- Key classes include flavonoids, tannins, lignins, and phenolic acids.
Purpose of the Study:
- To review methodologies for the analysis of phenolic compounds in plant-based products.
- To discuss recent technical developments in the quantification of phenolics.
- To provide an overview of extraction, identification, and quantification techniques.
Main Methods:
- Organic solvent extraction is the primary method for isolating phenolic compounds.
- Chemical procedures are employed for the detection of total phenolics.
- Spectrophotometric and chromatographic techniques are used for identifying and quantifying individual phenolic compounds.
Main Results:
- A comprehensive overview of analytical methodologies for phenolic compounds is presented.
- Recent advancements in quantification techniques are discussed.
- The review synthesizes information on various methods used in phenolic compound analysis.
Conclusions:
- Effective methodologies are crucial for understanding the role of phenolic compounds in plant-based products.
- Continued development in analytical techniques enhances the accurate quantification of phenolics.
- This review serves as a resource for researchers analyzing phenolic compounds.
