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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
Need for Obtaining Pure Cultures01:29

Need for Obtaining Pure Cultures

Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

Updated: May 13, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Cell isolation and culture.

Sihui Zhang1, Jeffrey R Kuhn

  • 1Department of Biological Sciences, Virginia Polytechnic Institute and State University, MC 0913, Blacksburg, VA 24061, USA.

Wormbook : the Online Review of C. Elegans Biology
|February 23, 2013
PubMed
Summary

This study provides efficient protocols for isolating and culturing Caenorhabditis elegans (C. elegans) cells from embryonic and larval stages. These methods enable detailed single-cell analysis for studying cell development and behavior.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Cell isolation and culture are fundamental for studying cellular functions under controlled conditions.
  • Advanced techniques allow for large-scale isolation and culture of primary C. elegans cells.
  • Isolated cells facilitate high-resolution microscopy and single-cell profiling.

Purpose of the Study:

  • To describe protocols for the isolation and culture of C. elegans embryonic and larval stage cells.
  • To enable researchers to perform detailed studies on cell-autonomous development and behavior.
  • To provide a foundation for various downstream applications like electrophysiology and microscopy.

Main Methods:

  • Protocols for isolating embryonic and L1 stage C. elegans cells from bacterial plates.

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Isolation and Culture Expansion of Tumor-specific Endothelial Cells
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Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

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Last Updated: May 13, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Isolation and Culture Expansion of Tumor-specific Endothelial Cells
10:15

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

  • Methods for isolating L2-L4 stage C. elegans cells from axenic liquid cultures.
  • Standard sterile cell culture techniques are detailed.

    • Main Results:

    • C. elegans embryonic and larval cells can be isolated within 3 hours.
    • Isolated cells remain viable for several days in culture.
    • The protocols are applicable to different developmental stages.

    Conclusions:

    • The described protocols offer efficient methods for C. elegans cell isolation and culture.
    • These techniques support advanced single-cell analyses, including development and behavior.
    • The study provides valuable resources for the C. elegans research community.