Related Experiment Video
Updated: May 13, 2026
11:44
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
Global microRNA elevation by inducible Exportin 5 regulates cell cycle entry.
Yuka W Iwasaki1, Kotaro Kiga, Hiroyuki Kayo
1Max-Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany.
RNA (New York, N.Y.)
|February 23, 2013
Summary
Global microRNA (miRNA) elevation is vital for cell cycle control. Exportin 5 (XPO5) induction drives this miRNA increase, ensuring proper cell proliferation and preventing defects.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Proper gene expression regulation during cell cycle entry is crucial for cell proliferation and cancer prevention.
- MicroRNA (miRNA) networks are emerging regulators of complex genetic pathways.
- Understanding miRNA involvement in cell cycle control is key to cellular regulation.
Purpose of the Study:
- To investigate the role of global microRNA (miRNA) elevation in controlling gene expression during cell cycle entry.
- To identify key molecular players involved in regulating miRNA levels during cell cycle progression.
- To elucidate the function of Exportin 5 (XPO5) in the context of cell cycle control and miRNA regulation.
Main Methods:
- Investigated miRNA expression levels during cell cycle entry.
- Examined the induction mechanism of Exportin 5 (XPO5) using PI3K pathway inhibitors.
- Assessed the impact of XPO5 inhibition on global miRNA levels and cell proliferation.
- Analyzed cell cycle progression, specifically the G1/S transition, following XPO5 manipulation.
Main Results:
- Global miRNA elevation was found to be critical for the gene expression program during cell cycle entry.
- Exportin 5 (XPO5) is rapidly induced upon cell cycle entry via a PI3K-dependent post-transcriptional mechanism.
- Inhibition of XPO5 induction led to impaired global miRNA elevation and a proliferation defect.
- XPO5 inhibition caused a delay in the G1/S phase transition, indicating a role in cell cycle progression.
Conclusions:
- Exportin 5 (XPO5) acts as a critical molecular hub controlling gene expression through global miRNA regulation during cell cycle entry.
- XPO5-mediated global miRNA elevation is essential for proper cell proliferation and timely cell cycle progression.
- The findings suggest XPO5 and its regulation of miRNAs are involved in diverse cellular events related to cell cycle control.
Related Concept Videos
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
02:38Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
02:38Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.