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Updated: May 13, 2026

07:16
DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
Published on: February 9, 2024
A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle
Qingwang Xue1, Lei Wang, Wei Jiang
1Key Laboratory for Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, PR China.
Abstract:
A novel label-free fluorescent sensing scheme for sensitive and selective detection of NAD(+) and ATP has been developed based on dumbbell probe-mediated rolling circle amplification (D-RCA)-responsive G-quadruplex formation. This approach can detect 0.5 pM for ATP and 100 fM for NAD(+), much lower than those of previously reported biosensors, and exhibits high discrimination ability.
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