[Control effect and bacteriostasis of mulberry endophytic bacterium Burkholderia cepacia Lu10-1 on silkworm...

A laboratory test was conducted to study the control effect and bacteriostasis of antagonistic bacterium Burkholderia cepacia Lu10-1 isolated from mulberry on silkworm septicemia, aimed to develop a new microbial pesticide to control silkworm diseases. The supernatant of Lu10-1 zymotic fluid achieved 41.2% control efficiency and 24.0% prophylactic effect on silkworm septicemia. The antibacterial crude extract of Lu10-1 had stronger antagonistic activity against Bacillus bombyseptieus. The diameter of inhibition zone reached 18.20 mm, and the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the antibacterial crude extract were 1.56 and 3.13 mg x mL(-1), respectively. After treated with the antibacterial crude extract, B. bombyseptieus never appeared logarithmic growth phase, its cell membrane permeability changed, intracellular protein leaked out, intracellular macromolecular protein degraded, and at last, the thalli cracked, inner substances out-flowed, cavity formed, and cell ablated. It was considered that the antagonistic substances of Lu10-1 strain could be used for controlling silkworm septicemia, with preferable development foreground.