Related Experiment Video
Updated: May 13, 2026
Use of Electromagnetic Navigational Transthoracic Needle Aspiration (E-TTNA) for Sampling of Lung Nodules
Published on: May 23, 2015
A prospective, comparative trial to optimize sampling techniques in EUS-guided FNA of solid pancreatic masses
Jun Kyu Lee1, Jong Hak Choi, Kwang Hyuck Lee
1Department of Internal Medicine, Dongguk University Ilsan Hospital, College of Medicine, Dongguk University, Seoul, Republic of Korea.
Background:
There is no standardization of the use of suction during puncturing of a target in pancreatic EUS-guided FNA (EUS-FNA). It is also debatable whether expressing aspirates from the needle by the traditional method of reinserting the stylet is more effective than by air flushing, which is easier and safer.
Objective:
To optimize sampling techniques in pancreatic EUS-FNA.
Design:
Prospective, comparative trial.
Setting:
Tertiary-care referral center.
Patients:
Eighty-one consecutive patients with solid pancreatic masses.
Intervention:
Four punctures were performed for each mass in random order by a 2 × 2 factorial design. Sample quality and diagnostic yield were compared between samples with suction (S+) versus no suction (S-) and expressed by reinserting the stylet (RS) versus air flushing (AF).
Main Outcome Measurements:
Sample quality by the number of diagnostic samples, cellularity, bloodiness, and air-drying artifact; diagnostic yield by accuracy, sensitivity, and specificity.
Results:
The number of diagnostic samples (72.8% vs 58.6%; P = .001), cellularity (odds ratio [OR] 2.12; 95% confidence interval [CI], 1.37-3.30; P < .001), bloodiness (OR 1.46; CI, 1.28-1.68; P < .001), accuracy (85.2% vs 75.9%; P = .004), and sensitivity (82.4% vs 72.1%; P = .005) were higher in S+ than in S-. Bloodiness was lower in AF than in RS (OR 1.16; CI, 1.03-1.30; P = .017).
Limitations:
Single-center trial, 2 kinds of needle gauges, and no immediate cytopathology evaluation.
Conclusion:
Puncturing with suction and expressing by air flushing may be used preferentially in pancreatic EUS-FNA because they were more effective and convenient techniques. (
Clinical Trial Registration Number:
NCT01354795.).
