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Published on: February 19, 2019
Anti-HBV active constituents from Piper longum
Zhi-Yong Jiang1, Wen-Feng Liu, Xue-Mei Zhang
1State Key Laboratory of Phytochemistry and Plant Resource in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, Yunnan, China. jiangzy2010@163.com
Ethanol extract of Piper longum Linn. showed potent anti-Hepatitis B virus (HBV) activity. Piperine demonstrated significant inhibition of HBsAg and HBeAg secretion, indicating its potential as an antiviral agent.
Area of Science:
- Phytochemistry
- Virology
- Medicinal Chemistry
Background:
- Hepatitis B virus (HBV) infection is a global health concern.
- Medicinal plants are a rich source of potential antiviral agents.
- Piper longum Linn. has been traditionally used for various ailments.
Purpose of the Study:
- To identify and isolate compounds from Piper longum Linn. with anti-HBV activity.
- To evaluate the in vitro efficacy of isolated compounds against HBV.
- To characterize novel compounds with potential therapeutic value.
Main Methods:
- Screening of ethanol extract of Piper longum Linn. for anti-HBV activity.
- Bioassay-guided fractionation and purification of active compounds.
- Structure elucidation using MS, IR, and NMR spectroscopic techniques.
- In vitro anti-HBV activity assay using Hep G 2.2.15 cell line.
Main Results:
- Four new compounds (two glycosides, two amide alkaloids) and two known compounds were isolated.
- Piperine (a known compound) exhibited remarkable inhibitory activity against HBsAg and HBeAg secretion.
- Selectivity Index (SI) values for piperine were 15.7 for HBsAg and 16.8 for HBeAg.
Conclusions:
- Piper longum Linn. is a valuable source of anti-HBV agents.
- Piperine shows significant potential for the development of new Hepatitis B therapies.
- Further research is warranted to explore the mechanism of action and therapeutic applications of these compounds.
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