Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effect of Medicaid coverage of tobacco-dependence treatments on smoking cessation.

International journal of environmental research and public health·2010

Same author

Cytokine and autoantibody patterns in acute liver failure.

Journal of immunotoxicology·2009

Same author

A novel scoring system for prognostic prediction in d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure BALB/c mice.

BMC gastroenterology·2009

Same author

Mammalian target of rapamycin signaling pathway contributes to glioma progression and patients' prognosis.

The Journal of surgical research·2009

Same author

Estrogen receptor neurobiology and its potential for translation into broad spectrum therapeutics for CNS disorders.

Current molecular pharmacology·2009

Same author

Transcriptional and post-translational regulation of adiponectin.

The Biochemical journal·2009

Search research articles

Related Experiment Video

Updated: May 13, 2026

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

Identification of somatic mutations in human prostate cancer by RNA-Seq.

Xiaolin Xu¹, KaiChang Zhu, Feng Liu

¹Department of Urinary Surgery, Fengxian Branch of Shanghai Sixth People's Hospital, Shanghai, 201499, China.

|February 26, 2013

Summary

RNA sequencing (RNA-Seq) identified 116 mutations in 92 genes from prostate cancer tissues. These findings highlight RNA-Seq

More Related Videos

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

Related Experiment Videos

Last Updated: May 13, 2026

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

Area of Science:

Genomics
Cancer Biology
Molecular Biology

Background:

RNA sequencing (RNA-Seq) is a powerful tool for transcriptome characterization.
Beyond gene expression, RNA-Seq can identify genetic variations within transcribed regions.
Prostate cancer research seeks novel genetic targets for diagnosis and treatment.

Purpose of the Study:

To investigate the utility of RNA-Seq for discovering somatic mutations in human prostate cancer.
To identify candidate genes with disruptive mutations in prostate cancer transcriptomes.
To evaluate the potential of identified mutations for cancer diagnosis and therapy.

Main Methods:

Analysis of transcriptome sequencing data from five human prostate cancer tissue samples.
Application of high-confidence variant calling to identify single nucleotide variants and indels.
Focus on disruptive mutations including frameshift indels and nonsynonymous nucleotide substitutions.

Main Results:

Identification of 116 high-confidence disruptive mutations across 92 genes.
Discovery of a frameshift insertion/deletion in the TNFSF10 gene, potentially impairing apoptosis induction.
Detection of other candidate genes with significant mutations relevant to prostate cancer.

Conclusions:

RNA-Seq is an effective method for somatic mutation screening in cancer.
The study provides a list of 92 candidate genes with potential roles in prostate cancer.
Identified mutations and genes offer prospects for improved prostate cancer diagnosis and treatment strategies.