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A novel reusable PAni-PVA-Amylase film: activity and analysis
Susmita Singh1, Jyoti Prasad Saikia, Alak Kumar Buragohain
1Department of Molecular Biology and Biotechnology, Tezpur (Central) University, Tezpur, P.O.-Napaam-784028, Sonitpur, Assam, India.
Abstract:
Alpha-amylase was immobilized onto polyaniline-polyvinyl alcohol (PAni-PVA) film by cross linking with glutaraldehyde. The activity yield and immobilization efficiency was calculated as 13.58% and 30.60% respectively. The PAni-PVA-Amylase films were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and amylase activity assay. The immobilized α-amylase had its optimum activity at pH 7.0 and 25°C. The immobilization of α-amylase onto the PAni-PVA films protected the enzyme from Fe(2+) inhibition. The PAni-PVA-Amylase films could be used effectively for 30 cycles with 60% retention of the activity.
