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Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Hematopoiesis01:21

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...

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Related Experiment Video

Updated: May 13, 2026

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Nrf2 regulates haematopoietic stem cell function.

Jennifer J Tsai1, Jarrod A Dudakov, Koichi Takahashi

  • 1Department of Immunology, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA.

Nature Cell Biology
|February 26, 2013
PubMed
Summary

Nuclear factor erythroid 2-related factor 2 (Nrf2) regulates hematopoietic stem cell (HSC) balance. Nrf2 deficiency causes HSC hyperproliferation, disrupting quiescence, self-renewal, and niche localization, impacting lifelong hematopoiesis.

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Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
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Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Published on: March 21, 2017

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Last Updated: May 13, 2026

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
11:06

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Published on: March 21, 2017

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Maintaining hematopoietic stem cell (HSC) quiescence, self-renewal, and proper bone marrow niche localization is vital for lifelong hematopoiesis.
  • HSC function is governed by intrinsic molecular mechanisms and extrinsic microenvironmental signaling.
  • Nuclear factor erythroid 2-related factor 2 (Nfe2l2, or Nrf2) is a known regulator of oxidative stress response.

Purpose of the Study:

  • To investigate the regulatory role of Nrf2 in HSC homeostasis.
  • To understand Nrf2's impact on HSC quiescence, self-renewal, and niche dynamics.
  • To identify novel molecular interactions involving Nrf2 in HSC maintenance.

Main Methods:

  • Analysis of Nrf2-deficient mouse models.
  • Assessment of hematopoietic stem and progenitor cell compartment size and proliferation.
  • Evaluation of HSC quiescence and self-renewal capacity.
  • Investigation of HSC migration and retention within bone marrow niches.
  • Exploration of the relationship between Nrf2 and CXCR4 signaling.

Main Results:

  • Nrf2 deficiency led to an expansion of the hematopoietic stem and progenitor cell compartment.
  • This expansion was driven by cell-intrinsic hyperproliferation at the expense of HSC quiescence and self-renewal.
  • Nrf2 was found to modulate both HSC migration and retention within their niche.
  • A novel link between Nrf2 and CXCR4 was identified, contributing to HSC function maintenance.

Conclusions:

  • Nrf2 plays a critical regulatory role in multiple aspects of HSC homeostasis.
  • Dysregulation of Nrf2 impacts HSC proliferation, quiescence, self-renewal, and niche localization.
  • The Nrf2-CXCR4 axis is a significant factor in maintaining HSC function.