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There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Osmoregulation in Fishes02:32

Osmoregulation in Fishes

When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
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Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
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Published on: March 18, 2016

Selenium content in seafood in Japan.

Yumiko Yamashita1, Michiaki Yamashita, Haruka Iida

  • 1National Research Institute of Fisheries Science, Kanazawa, Yokohama, Kanagawa, Japan. ymk0125@affrc.go.jp

Nutrients
|February 26, 2013
PubMed
Summary

Seafood is a rich source of selenium, an essential nutrient. Alfonsino muscle showed the highest selenium levels among tested seafood, with levels correlating to mercury in alfonsino tissues.

Area of Science:

  • Nutritional Science
  • Marine Biology
  • Environmental Health

Background:

  • Selenium is a vital micronutrient for human health.
  • Seafood, including fish and shellfish, is a primary dietary source of selenium.
  • Selenoneine, a selenium-containing imidazole compound, is prevalent in ocean fish muscle.

Purpose of the Study:

  • To quantify total selenium levels in commercially significant fish and shellfish species.
  • To identify seafood with the highest selenium concentrations.
  • To investigate the relationship between selenium and mercury levels in alfonsino.

Main Methods:

  • Measurement of total selenium concentrations in the edible portions of various seafood species.
  • Comparative analysis of selenium levels across different species and tissues.

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  • Correlation analysis between selenium and mercury levels in alfonsino tissues.
  • Main Results:

    • Alfonsino muscle exhibited the highest selenium concentration at 1.27 mg/kg.
    • High selenium levels (1.07–1.20 mg/kg) were detected in salted mullet and Pacific herring ovaries.
    • Selenium levels in other fish muscles ranged from 0.12 to 0.77 mg/kg.
    • A close correlation was observed between selenium and mercury levels in alfonsino muscles.
    • Selenium content varied significantly across different alfonsino tissues, ranging from 1.10 to 24.8 mg/kg.

    Conclusions:

    • Seafood, particularly alfonsino and certain fish roe products, represents a significant source of dietary selenium.
    • The co-occurrence of high selenium and mercury levels in some seafood warrants consideration for dietary recommendations.
    • Tissue-specific analysis reveals substantial variation in selenium accumulation within fish species like alfonsino.