Related Experiment Video
Updated: May 13, 2026
12:54
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
Published on: March 7, 2018
Preparation of cell lines for single-cell analysis of transcriptional activation dynamics
Ilona U Rafalska-Metcalf1, Susan M Janicki
1Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|February 26, 2013
Abstract:
Imaging molecularly defined regions of chromatin in single living cells during transcriptional activation has the potential to provide new insight into gene regulatory mechanisms. Here, we describe a method for isolating cell lines with multi-copy arrays of reporter transgenes, which can be used for real-time high-resolution imaging of transcriptional activation dynamics in single cells.
