Related Experiment Video

Updated: May 13, 2026

In ovo Expression of MicroRNA in Ventral Chick Midbrain
09:19

In ovo Expression of MicroRNA in Ventral Chick Midbrain

Published on: September 16, 2013

Expression of microRNA and microRNA processing machinery genes during early quail (Coturnix japonica) embryo

H Kocamis1, M Hossain, M U Cinar

  • 1Department of Histology and Embryology, Kirikkale University, Kirikkale, Turkey.

Poultry Science
|February 26, 2013
PubMed

Insights

MicroRNAs (miRNA) regulate key developmental processes. This study found 7 specific miRNAs differentially expressed during quail embryo somite formation, suggesting their critical roles in early development.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are small regulatory RNA molecules controlling vital physiological processes like cell division, differentiation, and organogenesis.
  • Understanding miRNA involvement in embryonic development is crucial for deciphering gene regulation during early life stages.

Purpose of the Study:

  • To investigate the expression patterns of 32 miRNAs and 18 miRNA processing genes during somite formation in quail embryos.
  • To identify specific miRNAs and their potential roles in early embryonic development and organogenesis.

Main Methods:

  • Quail embryos were collected at Hamburger and Hamilton stages 4, 6, and 9 (19, 24, and 30 hours of incubation).
  • Total RNA was isolated, and quantitative real-time PCR was employed to analyze the expression of selected miRNAs and miRNA processing machinery genes.

Main Results:

  • Seven miRNAs (let-7a, mir-122, mir-125b, mir-10b, let-7b, mir-26a, and mir-126) exhibited differential expression during early quail embryo development.
  • The expression of 18 miRNA processing machinery genes did not show significant changes at 30 hours compared to earlier stages.

Conclusions:

  • The functional miRNA biogenesis machinery suggests miRNAs are actively involved in regulating early quail development.
  • The identified 7 differentially expressed miRNAs are proposed to play significant roles in the critical process of quail embryo somite formation.

More Related Videos

Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm13:43

Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm

Published on: June 17, 2018

Ecotoxicological Effects of Microplastics on Bird Embryo Development by Hatching without Eggshell08:11

Ecotoxicological Effects of Microplastics on Bird Embryo Development by Hatching without Eggshell

Published on: August 14, 2021

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...