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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

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Related Experiment Video

Updated: May 13, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Mass spectrometry-based methods to study protein architecture and dynamics.

Igor A Kaltashov1, Cedric E Bobst, Rinat R Abzalimov

  • 1Department of Chemistry, University of Massachusetts-Amherst, Amherst, Massachusetts, USA. Kaltashov@chem.umass.edu

Protein Science : a Publication of the Protein Society
|February 26, 2013
PubMed
Summary

Mass spectrometry is a vital tool in molecular biophysics for analyzing protein structure beyond basic sequencing. This review focuses on hydrogen exchange and electrospray ionization mass spectrometry for advanced protein structural studies.

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Analyzing Large Protein Complexes by Structural Mass Spectrometry
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Analyzing Large Protein Complexes by Structural Mass Spectrometry

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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

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Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

Area of Science:

  • Molecular biophysics
  • Biochemistry
  • Analytical chemistry

Background:

  • Mass spectrometry is crucial for diverse molecular biophysics applications.
  • It is used for protein sequencing, modification analysis, and structural studies.

Purpose of the Study:

  • To review popular mass spectrometry tools for protein structural analysis.
  • Emphasis on techniques beyond covalent structure determination.

Main Methods:

  • Hydrogen exchange mass spectrometry (HX-MS)
  • Direct electrospray ionization mass spectrometry (ESI-MS)
  • Analysis of higher-order protein structure

Main Results:

  • HX-MS and ESI-MS are powerful for studying protein structure and dynamics.
  • These methods provide insights into protein folding, stability, and interactions.
  • Mass spectrometry offers a versatile platform for comprehensive protein analysis.

Conclusions:

  • Mass spectrometry is indispensable for advanced protein structural biology.
  • Hydrogen exchange and direct ESI-MS are key techniques for exploring protein structure.
  • These methods advance our understanding of protein function and behavior.