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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 13, 2026

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
12:53

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Published on: July 6, 2014

Initial quantitative proteomic map of 28 mouse tissues using the SILAC mouse.

Tamar Geiger1, Ana Velic, Boris Macek

  • 1Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.

Molecular & Cellular Proteomics : MCP
|February 26, 2013
PubMed
Summary

This study presents a comprehensive proteomic atlas of 28 mouse tissues, revealing how protein expression defines tissue function and specialization. The findings highlight tissue-specific protein profiles and offer a framework for correlating proteomes with physiological roles.

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Related Experiment Videos

Last Updated: May 13, 2026

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
12:53

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Published on: July 6, 2014

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
07:54

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
08:13

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Published on: April 9, 2019

Area of Science:

  • Proteomics
  • Mammalian Biology
  • Bioinformatics

Background:

  • Understanding mammalian tissue function requires identifying tissue-specific proteins.
  • Global and quantitative proteome analysis is crucial for a whole-organism perspective.

Purpose of the Study:

  • To create a comprehensive proteomic atlas of 28 mouse tissues.
  • To analyze tissue-specific protein expression and its relation to physiological functions.

Main Methods:

  • Proteomic analysis of 28 mouse tissues using high-resolution mass spectrometry.
  • Stable isotope labeling with amino acids in cell culture (SILAC) as an internal standard for accurate quantification.
  • Bioinformatic analysis of proteomic data.

Main Results:

  • Identification of 7,349 proteins and quantification of 6,974 across 28 mouse tissues.
  • Physiologically related tissues clustered together based on proteomic profiles.
  • Highly expressed proteins characterize tissue functions, with specialization evident in the top 100 most abundant proteins.

Conclusions:

  • Proteomic profiles reflect tissue specialization, even for broadly expressed proteins like ribosomal and spliceosomal components.
  • A computational framework was developed to correlate proteome profiles with tissue physiological functions.
  • The generated atlas serves as a valuable resource for the scientific community studying mammalian protein expression.