Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromosome 22q13 terminal deletion size is associated with relevant clinical features in a sample of 63 Italian patients with Phelan-McDermid syndrome.

Journal of neurodevelopmental disorders·2026
Same author

Unveiling the Molecular Mechanism of Intestinal Metabolite para-Cresol in Modulating Neuroinflammation and Synaptic Dysfunction: Implications for Autism Spectrum Disorder.

Journal of neurochemistry·2026
Same author

Frequency-specific electromagnetic modulation of brain redox state precedes cortical activity in stroke recovery.

iScience·2026
Same author

Lipid membrane remodeling by myristic acid treatment reverses Parkinson's disease α-synuclein phenotypes in patient neurons.

npj metabolic health and disease·2026
Same author

The Role of Microbiota Metabolites Propionic Acid, p-Cresol, and 4-Ethylphenyl Sulfate in Autism Susceptibility: A Systematic Review.

Autism research : official journal of the International Society for Autism Research·2026
Same author

Family history enrichment in Non-Small cell Lung Cancer: A cross-sectional - prospective study to inform referral for germline testing.

Lung cancer (Amsterdam, Netherlands)·2026
Same journal

Analysis of strength degradation of coal and rock masses and stability of mined areas under long term immersion environment.

PloS one·2026
Same journal

Biogenic Silver-Selenium nanocomposite with anticancer activity and potent efficacy against vancomycin-resistant Staphylococcus aureus.

PloS one·2026
Same journal

Preparation and physicochemical characterization of a biodegradable chitosan/carboxymethyl cellulose hydrogel synthesized in NaOH/urea medium.

PloS one·2026
Same journal

Action-guilt, survivor-guilt, and depression in combat-related PTSD.

PloS one·2026
Same journal

Explainable machine learning for predicting activities of daily living at discharge in stroke patients: A retrospective study using SHAP interpretability.

PloS one·2026
Same journal

Deep learning based two-way feature depiction model for brain tumor detection.

PloS one·2026
See all related articles

Related Experiment Video

Updated: May 13, 2026

Dynamic Clamp Methods to Investigate Impaired Neuronal Excitability Associated with Autism
08:44

Dynamic Clamp Methods to Investigate Impaired Neuronal Excitability Associated with Autism

Published on: October 17, 2025

Age-dependent decrease and alternative splicing of methionine synthase mRNA in human cerebral cortex and an

Christina R Muratore1, Nathaniel W Hodgson, Malav S Trivedi

  • 1Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University, Boston, MA, USA.

Plos One
|February 26, 2013
PubMed
Summary
This summary is machine-generated.

Methionine synthase (MS) enzyme activity decreases significantly with age and in autism. This decline impacts brain metabolism and methylation, potentially increasing neurological disorder risk.

More Related Videos

Generation and Characterization of Human Induced Pluripotent Stem Cell-derived Astrocytes Lacking Fragile X Messenger Ribonucleoprotein
10:59

Generation and Characterization of Human Induced Pluripotent Stem Cell-derived Astrocytes Lacking Fragile X Messenger Ribonucleoprotein

Published on: June 6, 2025

Hypoxia Alters miRNAs Levels Involved in Non-Mendelian Inheritance of Autism Spectrum Disorder in Mice
09:13

Hypoxia Alters miRNAs Levels Involved in Non-Mendelian Inheritance of Autism Spectrum Disorder in Mice

Published on: July 11, 2025

Related Experiment Videos

Last Updated: May 13, 2026

Dynamic Clamp Methods to Investigate Impaired Neuronal Excitability Associated with Autism
08:44

Dynamic Clamp Methods to Investigate Impaired Neuronal Excitability Associated with Autism

Published on: October 17, 2025

Generation and Characterization of Human Induced Pluripotent Stem Cell-derived Astrocytes Lacking Fragile X Messenger Ribonucleoprotein
10:59

Generation and Characterization of Human Induced Pluripotent Stem Cell-derived Astrocytes Lacking Fragile X Messenger Ribonucleoprotein

Published on: June 6, 2025

Hypoxia Alters miRNAs Levels Involved in Non-Mendelian Inheritance of Autism Spectrum Disorder in Mice
09:13

Hypoxia Alters miRNAs Levels Involved in Non-Mendelian Inheritance of Autism Spectrum Disorder in Mice

Published on: July 11, 2025

Area of Science:

  • Neuroscience
  • Biochemistry
  • Genetics

Background:

  • Methionine synthase (MS) is a critical enzyme dependent on folate and vitamin B12.
  • MS activity is sensitive to oxidative stress, influencing glutathione production and methylation reactions.
  • Methylation is vital for metabolic activity and epigenetic regulation.

Purpose of the Study:

  • To investigate age-related changes in methionine synthase (MS) mRNA and protein levels in the human brain.
  • To explore the impact of alternative splicing on MS function.
  • To examine the association between MS mRNA levels, autism, and oxidative stress markers.

Main Methods:

  • Quantification of MS mRNA levels in postmortem human cortical tissue across the lifespan.
  • Analysis of MS alternative splicing variants.
  • Measurement of MS protein and methionine levels.
  • Comparison of MS mRNA levels in autistic versus neurotypical subjects.
  • In vitro studies using cultured neuronal cells treated with TNF-α.

Main Results:

  • MS mRNA levels showed a dramatic biphasic decrease from gestation to 84 years.
  • Alternative splicing of MS mRNA, including exon deletions, was observed and varied with age.
  • MS mRNA levels were significantly lower in autistic subjects, particularly at younger ages.
  • Decreased MS mRNA levels were replicated in neuronal cells treated with TNF-α.
  • No significant changes in MS protein or methionine levels correlated with decreasing mRNA.

Conclusions:

  • Methionine synthase plays a dynamic role in brain metabolism and development, not just a housekeeping function.
  • Age-related decline and reduced MS mRNA levels in autism suggest a link to oxidative stress.
  • Dysregulation of MS may contribute to neurological disorders through altered methylation and epigenetic processes.