Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 13, 2026

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
05:40

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation

Published on: September 8, 2016

Differential gene expression profiling and biological process analysis in proximal nerve segments after sciatic nerve

Shiying Li1, Qianqian Liu, Yongjun Wang

  • 1Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China.

Plos One
|February 26, 2013
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiomic data integration unveils molecular mechanisms underlying chestnut and skin tissue differentiation in Dezhou donkeys.

BMC genomics·2026
Same author

Liquid biopsy-based multi-omics approach for early detection of hepatocellular carcinoma (ASCEND-Hep): a multiphase prospective development and validation study.

BMC medicine·2026
Same author

DCAF7 enhances atherosclerosis in vascular endothelial cells by promoting SLC40A1 transcription through facilitating TNF-mediated NF-κB function.

Biochemistry and biophysics reports·2026
Same author

Ancestral DOF27 and GDH1 haplotypes drive rice adaptation to ammonium habitats.

Current biology : CB·2026
Same author

FK506-binding proteins OsFKBP57 and OsFKBP73 confer salt tolerance in rice by activating the SOS pathway while reducing grain protein accumulation through inhibition of BCAT activity.

Plant communications·2026
Same author

Mannose-binding lectin 2 secreted by hepatocellular carcinoma cells recruits and activates natural killer cells to reshape an immune-activated microenvironment.

PLoS biology·2026

Peripheral nerve regeneration involves dynamic gene expression changes. This study profiles mRNA changes after sciatic nerve injury in rats, revealing key regulatory genes and biological processes, including novel olfactory receptor findings.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Regenerative Medicine

Background:

  • Peripheral nerve injury triggers complex regeneration involving cellular and molecular factors.
  • Global gene expression changes during nerve regeneration remain incompletely understood.
  • Existing knowledge lacks comprehensive profiling of molecular events post-injury.

Purpose of the Study:

  • To comprehensively profile global messenger RNA (mRNA) expression changes in rat sciatic nerves following transection.
  • To identify key biological processes and regulatory genes involved in peripheral nerve regeneration.
  • To investigate temporal expression patterns of genes at various time points post-injury.

Main Methods:

  • Sciatic nerve transection model in adult rats.

More Related Videos

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis
08:49

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis

Published on: February 22, 2012

Related Experiment Videos

Last Updated: May 13, 2026

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
05:40

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation

Published on: September 8, 2016

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis
08:49

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis

Published on: February 22, 2012

  • DNA microarray analysis to profile global mRNA expression changes at multiple time points (0.5 hours to 14 days).
  • Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) for gene expression validation.
  • Gene ontology enrichment analysis to identify involved biological pathways.

    • Main Results:

    • A large number of genes exhibited differential expression across various time points post-sciatic nerve transection.
    • Distinct temporal expression patterns were observed for numerous genes, highlighting dynamic molecular changes.
    • Gene ontology analysis revealed significant shifts in biological processes during nerve regeneration.
    • Olfactory receptors were identified in sciatic nerves for the first time.

    Conclusions:

    • Peripheral nerve injury induces dynamic changes in biological processes and time-dependent expression of key regulatory genes.
    • This study provides a molecular-level perspective on nerve regeneration.
    • The discovery of olfactory receptors in sciatic nerves opens new avenues for research.