[High performance liquid chromatography-validated analytical method for 1% permethrin quantification].

Area of Science:

• Analytical Chemistry
• Pharmacology
• Public Health Entomology

Context:

• Increasing prevalence of Pediculus capitis (head lice) infestations globally necessitates effective control programs.
• Synthetic pyrethroids, such as permethrin, are widely used pediculicides due to their efficacy and favorable safety profile.
• Quality control of pharmaceutical preparations is essential to ensure product safety and therapeutic effectiveness.

Purpose:

• To validate an analytical method using high-performance liquid chromatography (HPLC) for quantifying 1% permethrin pediculicidal preparations.
• To assess the accuracy, precision, and linearity of the HPLC method for permethrin quantification.
• To apply the validated method for analyzing Cuban-made 1% permethrin pediculicide batches.

Summary:

• A high-performance liquid chromatography (HPLC) method was validated for the quantification of permethrin isomers in pediculicidal solutions.
• The method demonstrated excellent linearity (correlation coefficient > 0.99), precision (variation coefficient < 3%), and accuracy (relative error within ±3%).
• Analysis of two batches of 1% permethrin solution revealed a mean concentration of 6780.14 µg/mL, which was below the expected range.

Impact:

• The validated HPLC method provides a reliable tool for quality control of 1% permethrin pediculicide products.
• Ensuring the quality of permethrin preparations contributes to the effective management of Pediculus capitis infestations.
• This analytical approach supports public health initiatives by guaranteeing the potency of pediculicidal treatments.