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Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model
Published on: December 20, 2018
[Phenotypic characterization of Aeromonas isolated from patients with acute diarrheal diseases]
Laura Bravo Fariñas1, Anabel Fernández Abreu, Deyánira González Leiva
1Instituto de Medicina Tropical "Pedro Kouri (IPK), La Habana, Cuba. laura@ipk.sld.cu
Insights
Aeromonas caviae and Aeromonas hydrophila are the most common causes of diarrhea in Cuba. All strains showed antimicrobial resistance, highlighting the need for effective treatment options like tetracycline and chloramphenicol.
Area of Science:
- Microbiology
- Infectious Diseases
- Public Health
Context:
- Acute diarrheal diseases are a major cause of illness and death in young children globally.
- Antimicrobial resistance is a growing public health concern, complicating treatment.
- Aeromonas species are emerging as significant pathogens in diarrheal diseases.
Purpose:
- To identify the most common Aeromonas species causing diarrhea in Cuban patients.
- To assess the antimicrobial susceptibility patterns of these isolated Aeromonas species.
Summary:
- Aeromonas caviae (33%) and Aeromonas hydrophila (29%) were the most frequently identified species in diarrheal fecal samples.
- All 100 isolates exhibited resistance to at least one tested antimicrobial.
- Highest resistance rates were observed for cefalotine, sulfonamides, and nalidixic acid.
Impact:
- The study identifies key Aeromonas species contributing to diarrheal diseases in Cuba.
- It underscores the widespread antimicrobial resistance among these pathogens.
- Tetracycline and chloramphenicol are recommended as effective treatments for Aeromonas-related intestinal infections in the region.
Introduction:
The acute diarrheal diseases are one of the main causes of morbidity and mortality in children aged under 5 years and in the general population; this demands a great deal of care in the healthcare services. The situation worsens due to the overuse of antimicrobials and the development of bacterial resistance, being the latter an emerging health problem in different areas of the world. Among the causative microorganisms of acute diarrheal diseases are those of Aeromonas genus, recognized as second risk emerging pathogens.
Objectives:
To determine the most frequently isolated Aeromonas species in fecal samples from acute diarrheal patients and their antimicrobial susceptibility.
Methods:
The Bauer-Kirby's method allowed identifying the susceptibility to several antimicrobials of 100 isolated samples coming from the provincial hygiene and epidemiology centers during 2007 and 2008.
Results:
Identification of the species was accomplished in 67% of isolates, being A. caviae (33%) y A. hydrophila (29%) the predominant species. It was demonstrated that 100% of isolates got resistant to at least one of the studied antimicrobials. Drug resistance to cefalotine, sulfonamides and nalidixic acid showed the highest percentages.
Conclusions:
Tetracycline and chloramphenicol are recommended as the drugs of choice for treating intestinal infections caused by these microorganisms in Cuba.
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